› 2zqr

X-ray diffraction
2.5Å resolution

Crystal structure of AUH without RNA

Released:
DOI: 10.2210/pdb2zqr/pdb
PDB entry 2zqr coloured by chain, front view.
PDB entry 2zqr coloured by chain, side view.
PDB entry 2zqr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
AU-rich RNA-binding induces changes in the quaternary structure of AUH.
Kurimoto K, Kuwasako K, Sandercock AM, Unzai S, Robinson CV, Muto Y, Yokoyama S
Proteins 75 360-72 (2009)
PMID: 18831052

Function and Biology Details

Reactions catalysed: 
(S)-3-hydroxy-3-methylglutaryl-CoA = trans-3-methylglutaconyl-CoA + H(2)O
Citramalyl-CoA = itaconyl-CoA + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylglutaconyl-CoA hydratase, mitochondrial Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 272 amino acids
Theoretical weight: 29.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13825 (Residues: 68-339; Coverage: 80%)
Gene name: AUH
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 79.208Å b: 132.429Å c: 80.043Å
α: 90° β: 108.1° γ: 90°
R-values:
R R work R free
0.197 0.197 0.263
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Metabolic biology of 3-methylglutaconic acid-uria: a new perspective.
Su et al. (2014)
5 other articles

2 mentions without citation

The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family.
Poudel et al. (2015)
1 more