› 2ziz

X-ray diffraction
2.2Å resolution

Crystal structure of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with NAD and 3-deazaadenosine

Released:
DOI: 10.2210/pdb2ziz/pdb
PDB entry 2ziz coloured by chain, front view.
PDB entry 2ziz coloured by chain, side view.
PDB entry 2ziz coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors.
Reddy MC, Kuppan G, Shetty ND, Owen JL, Ioerger TR, Sacchettini JC
Protein Sci 17 2134-44 (2008)
PMID: 18815415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 495 amino acids
Theoretical weight: 54.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGV3 (Residues: 2-495; Coverage: 100%)
Gene names: MTCY20B11.23c, Rv3248c, ahcY, sahH
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AD3 4 x AD3
Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 100.55Å b: 111.317Å c: 94.473Å
α: 90° β: 96.77° γ: 90°
R-values:
R R work R free
0.202 0.198 0.285
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phylogenetic framework and molecular signatures for the main clades of the phylum Actinobacteria.
Gao et al. (2012)
4 more

5 mentions without citation

S-adenosyl-L-homocysteine hydrolase and methylation disorders: yeast as a model system.
Tehlivets et al. (2013)
4 more