› 2zfd

X-ray diffraction
1.2Å resolution

The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14

Released:
DOI: 10.2210/pdb2zfd/pdb
PDB entry 2zfd coloured by chain, front view.
PDB entry 2zfd coloured by chain, side view.
PDB entry 2zfd coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14.
Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, Shimizu T
J Mol Biol 377 246-57 (2008)
PMID: 18237745

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcineurin B-like protein 2 Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8LAS7 (Residues: 1-226; Coverage: 100%)
Gene names: At5g55990, CBL2, MDA7.3, SCABP1
Sequence domains: EF hand
Structure domains: EF-hand
CBL-interacting serine/threonine-protein kinase 14 Chain: B
Molecule details ›
Chain: B
Length: 123 amino acids
Theoretical weight: 14.06 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LZW4 (Residues: 305-427; Coverage: 28%)
Gene names: At5g01820, CIPK14, PKS24, SR1, SnRK3.15, T20L15.90
Sequence domains: NAF domain
Structure domains: Kinase associated domain 1, KA1

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 34.092Å b: 45.481Å c: 92.39Å
α: 90° β: 92.64° γ: 90°
R-values:
R R work R free
0.18 0.18 0.192
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The CBL-CIPK network in plant calcium signaling.
Luan S. (2009)
12 more

1 mention without citation

Root angle in maize influences nitrogen capture and is regulated by calcineurin B-like protein (CBL)-interacting serine/threonine-protein kinase 15 (ZmCIPK15).
Schneider et al. (2022)