Function and Biology Details
Reaction catalysed:
dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107275 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-alpha-mycarosylerythronolide B desosaminyl transferase
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 48.15 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 441 amino acids
Theoretical weight: 48.15 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
A4F7P3 (Residues: 1-421; Coverage: 100%)
Sequence domains:
- Erythromycin biosynthesis protein CIII-like, N-terminal domain
- Erythromycin biosynthesis protein CIII-like, C-terminal domain
Cytochrome P450 family protein EryCII
Molecule details ›
Chain: B
Length: 381 amino acids
Theoretical weight: 40.73 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450
Length: 381 amino acids
Theoretical weight: 40.73 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: A4F7P2 (Residues: 1-361; Coverage: 100%)
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID29
Spacegroup:
P23
Expression system: Escherichia coli BL21(DE3)
