› 2yfb

X-ray diffraction
1.9Å resolution

X-ray structure of McpS ligand binding domain in complex with succinate

Released:
DOI: 10.2210/pdb2yfb/pdb
PDB entry 2yfb coloured by chain, front view.
PDB entry 2yfb coloured by chain, side view.
PDB entry 2yfb coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Primary publication:
Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites.
Pineda-Molina E, Reyes-Darias JA, Lacal J, Ramos JL, García-Ruiz JM, Gavira JA, Krell T
Proc Natl Acad Sci U S A 109 18926-31 (2012)
PMID: 23112148

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein McpS Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.65 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88E10 (Residues: 46-283; Coverage: 37%)
Gene names: PP_4658, mcpS
Sequence domains: Helical bimodular sensor domain
Structure domains: de novo design (two linked rop proteins)

Ligands and Environments

3 bound ligands:
Ligand SIN 2 x SIN
Ligand ACT 2 x ACT
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: C2
Unit cell:
a: 226.067Å b: 46.039Å c: 50.823Å
α: 90° β: 95.92° γ: 90°
R-values:
R R work R free
0.207 0.204 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Pseudomonas chemotaxis.
Sampedro et al. (2015)
9 more

5 mentions without citation

Sensory Repertoire of Bacterial Chemoreceptors.
Ortega et al. (2017)
4 more