› 2ye1

X-ray diffraction
1.63Å resolution

X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)

Released:
DOI: 10.2210/pdb2ye1/pdb
PDB entry 2ye1 coloured by chain, front view.
PDB entry 2ye1 coloured by chain, side view.
PDB entry 2ye1 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TWJ, Royant A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.62 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand SWG 1 x SWG

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.14Å b: 61.802Å c: 69.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.194
Expression system: Escherichia coli BL21(DE3)

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