› 2y8e

X-ray diffraction
1.39Å resolution

Crystal structure of D. melanogaster Rab6 GTPase bound to GMPPNP

Released:
DOI: 10.2210/pdb2y8e/pdb
PDB entry 2y8e coloured by chain, front view.
PDB entry 2y8e coloured by chain, side view.
PDB entry 2y8e coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of the Drosophila melanogaster Rab6 GTPase at 1.4 Å resolution.
Walden M, Jenkins HT, Edwards TA
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 744-8 (2011)
PMID: 21795785

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-127574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.34 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O18334 (Residues: 1-177; Coverage: 85%)
Gene names: CG6601, Rab6, wrt
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GNP 2 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 116.52Å b: 42.71Å c: 86.86Å
α: 90° β: 133.12° γ: 90°
R-values:
R R work R free
0.146 0.145 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Comparative modeling of Rab6 proteins: identification of key residues and their interactions with guanine nucleotides.
Mulukala Narasimha et al. (2013)

4 mentions without citation

Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein.
Liu et al. (2013)
3 more