› 2xys

X-ray diffraction
1.91Å resolution

Crystal structure of Aplysia californica AChBP in complex with strychnine

Released:
DOI: 10.2210/pdb2xys/pdb
PDB entry 2xys coloured by chain, front view.
PDB entry 2xys coloured by chain, side view.
PDB entry 2xys coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
A structural and mutagenic blueprint for molecular recognition of strychnine and d-tubocurarine by different cys-loop receptors.
Brams M, Pandya A, Kuzmin D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C
PLoS Biol 9 e1001034 (2011)
PMID: 21468359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Aplysia californica
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WSF8 (Residues: 20-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand SY9 6 x SY9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 100.593Å b: 100.593Å c: 289.676Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Structure and pharmacology of pentameric receptor channels: from bacteria to brain.
Corringer et al. (2012)
11 more

11 mentions without citation

Structure-Function of Neuronal Nicotinic Acetylcholine Receptor Inhibitors Derived From Natural Toxins.
Ho et al. (2020)
10 more