› 2xur

X-ray diffraction
1.9Å resolution

The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication

Released:
DOI: 10.2210/pdb2xur/pdb
PDB entry 2xur coloured by chain, front view.
PDB entry 2xur coloured by chain, side view.
PDB entry 2xur coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication.
Johnsen L, Flåtten I, Dalhus B, Bjørås M, Waldminghaus T, Skarstad K
Mol Microbiol 79 433-46 (2011)
PMID: 21219462

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 41.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 79.981Å b: 66.487Å c: 81.699Å
α: 90° β: 113.75° γ: 90°
R-values:
R R work R free
0.197 0.194 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Regulating DNA replication in bacteria.
Skarstad et al. (2013)
1 more