› 2xsw

X-ray diffraction
1.9Å resolution

Crystal structure of human INPP5E

Released:
DOI: 10.2210/pdb2xsw/pdb
PDB entry 2xsw coloured by chain, front view.
PDB entry 2xsw coloured by chain, side view.
PDB entry 2xsw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tresaugues L, Schutz P, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson I, Karlberg T, Kol S, Kotenyova T, Kouznetsova E, Moche M, Nyman T, Persson C, Schuler H, Schutz P, Siponen MI, Thorsell AG, Van Der Berg S, Wahlberg E, Weigelt J, Welin M, Nordlund P

Function and Biology Details

Reactions catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 3,4-diphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol polyphosphate 5-phosphatase type IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 40.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NRR6 (Residues: 275-623; Coverage: 54%)
Gene name: INPP5E
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 50.528Å b: 103.579Å c: 137.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.195
Expression system: Escherichia coli BL21(DE3)

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