› 2xna

X-ray diffraction
2.1Å resolution

Crystal structure of the complex between human T cell receptor and staphylococcal enterotoxin

Released:
DOI: 10.2210/pdb2xna/pdb
PDB entry 2xna coloured by chain, front view.
PDB entry 2xna coloured by chain, side view.
PDB entry 2xna coloured by chain, top view.
Source organisms:
Primary publication:
The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation.
Saline M, Rödström KE, Fischer G, Orekhov VY, Karlsson BG, Lindkvist-Petersson K
Nat Commun 1 119 (2010)
PMID: 21081917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134737 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
T cell receptor alpha chain constant Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 27.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Enterotoxin type H Chain: C
Molecule details ›
Chain: C
Length: 217 amino acids
Theoretical weight: 25.18 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A0M0 (Residues: 25-241; Coverage: 100%)
Gene names: entH, seh
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 182.692Å b: 49.98Å c: 132.834Å
α: 90° β: 131.61° γ: 90°
R-values:
R R work R free
0.225 0.223 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12

Quick links

Citations

12 review citations

Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning.
Fisher et al. (2018)
11 more

4 mentions without citation

Single-molecule spectroscopy and imaging over the decades.
Moerner et al. (2015)
3 more