› 2xjp

X-ray diffraction
0.95Å resolution

X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and mannose

Released:
DOI: 10.2210/pdb2xjp/pdb
PDB entry 2xjp coloured by chain, front view.
PDB entry 2xjp coloured by chain, side view.
PDB entry 2xjp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of flocculin-mediated social behavior in yeast.
Veelders M, Brückner S, Ott D, Unverzagt C, Mösch HU, Essen LO
Proc Natl Acad Sci U S A 107 22511-6 (2010)
PMID: 21149680

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flocculation protein FLO5 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38894 (Residues: 23-271; Coverage: 24%)
Gene names: FLO5, YHR211W
Sequence domains: PA14 domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand MAN 1 x MAN
Ligand BMA 2 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 46.28Å b: 61.82Å c: 106.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.106 0.105 0.122
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall.
Orlean P. (2012)
13 more

13 mentions without citation

Man-Specific Lectins from Plants, Fungi, Algae and Cyanobacteria, as Potential Blockers for SARS-CoV, MERS-CoV and SARS-CoV-2 (COVID-19) Coronaviruses: Biomedical Perspectives.
Barre et al. (2021)
12 more