› 2xj7

X-ray diffraction
2Å resolution

BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine

Released:
DOI: 10.2210/pdb2xj7/pdb
PDB entry 2xj7 coloured by chain, front view.
PDB entry 2xj7 coloured by chain, side view.
PDB entry 2xj7 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Inhibition of O-GlcNAcase using a potent and cell-permeable inhibitor does not induce insulin resistance in 3T3-L1 adipocytes.
Macauley MS, He Y, Gloster TM, Stubbs KA, Davies GJ, Vocadlo DJ
Chem Biol 17 937-48 (2010)
PMID: 20851343

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase BT_4395 Chains: A, B
Molecule details ›
Chains: A, B
Length: 716 amino acids
Theoretical weight: 82.32 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q89ZI2 (Residues: 22-737; Coverage: 100%)
Gene name: BT_4395
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GC2 2 x GC2
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 51.493Å b: 93.981Å c: 98.815Å
α: 104.13° β: 93.88° γ: 103.13°
R-values:
R R work R free
0.205 0.203 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Protein O-GlcNAcylation: emerging mechanisms and functions.
Yang et al. (2017)
15 more

4 mentions without citation

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis.
Fang et al. (2021)
3 more