› 2xgd

X-ray diffraction
2.25Å resolution

Crystal structure of a designed homodimeric variant T-A(L)A(L) of the tetracycline repressor

Released:
DOI: 10.2210/pdb2xgd/pdb
PDB entry 2xgd coloured by chain, front view.
PDB entry 2xgd coloured by chain, side view.
PDB entry 2xgd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Computational design of a chain-specific tetracycline repressor heterodimer.
Stiebritz MT, Wengrzik S, Klein DL, Richter JP, Srebrzynski A, Weiler S, Muller YA
J Mol Biol 403 371-85 (2010)
PMID: 20816982

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class B from transposon Tn10; Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04483 (Residues: 1-56; Coverage: 27%)
  • Canonical: P0ACT4 (Residues: 57-208; Coverage: 70%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I4122
Unit cell:
a: 69.656Å b: 69.656Å c: 184.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Status quo of tet regulation in bacteria.
Bertram et al. (2022)
4 other articles