› 2xet

X-ray diffraction
1.6Å resolution

Conserved hydrophobic clusters on the surface of the Caf1A usher C-terminal domain are important for F1 antigen assembly

Released:
DOI: 10.2210/pdb2xet/pdb
PDB entry 2xet coloured by chain, front view.
PDB entry 2xet coloured by chain, side view.
PDB entry 2xet coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Conserved hydrophobic clusters on the surface of the Caf1A usher C-terminal domain are important for F1 antigen assembly.
Dubnovitsky AP, Duck Z, Kersley JE, Härd T, MacIntyre S, Knight SD
J Mol Biol 403 243-59 (2010)
PMID: 20797400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F1 capsule-anchoring protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 89 amino acids
Theoretical weight: 9.55 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26949 (Residues: 745-833; Coverage: 11%)
Gene names: Y1099, YPMT1.83, YP_pMT083, caf1A
Sequence domains: PapC C-terminal domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1, ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 39.669Å b: 40.336Å c: 44.862Å
α: 90° β: 92.69° γ: 90°
R-values:
R R work R free
0.168 0.167 0.209
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Diversity in Genetic Regulation of Bacterial Fimbriae Assembled by the Chaperone Usher Pathway.
Gahlot et al. (2022)
5 other articles