› 2x9j

X-ray diffraction
1.85Å resolution

Structure of the Mutant D206N of Phycoerythrobilin Synthase PebS from the Cyanophage P-SSM2 in complex with bound substrate Biliverdin IXA

Released:
DOI: 10.2210/pdb2x9j/pdb
PDB entry 2x9j coloured by chain, front view.
PDB entry 2x9j coloured by chain, side view.
PDB entry 2x9j coloured by chain, top view.
Source organism: Prochlorococcus phage P-SSM2
Primary publication:
Radical mechanism of cyanophage phycoerythrobilin synthase (PebS).
Busch AW, Reijerse EJ, Lubitz W, Hofmann E, Frankenberg-Dinkel N
Biochem J 433 469-76 (2011)
PMID: 21050180

Function and Biology Details

Reaction catalysed: 
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin = biliverdin IX-alpha + 2 reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phycoerythrobilin synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 27.29 KDa
Source organism: Prochlorococcus phage P-SSM2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58MU6 (Residues: 1-233; Coverage: 100%)
Gene names: PSSM2_058, pebS
Sequence domains: Ferredoxin-dependent bilin reductase
Structure domains: oxygen-dependent coproporphyrinogen oxidase

Ligands and Environments

1 bound ligand:
Ligand BLA 2 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 53.81Å b: 63.15Å c: 81.65Å
α: 90° β: 108.53° γ: 90°
R-values:
R R work R free
0.159 0.157 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Shedding new light on viral photosynthesis.
Puxty et al. (2015)
1 more