› 2x6q

X-ray diffraction
2.2Å resolution

Crystal structure of trehalose synthase TreT from P.horikoshi

Released:
DOI: 10.2210/pdb2x6q/pdb
PDB entry 2x6q coloured by chain, front view.
PDB entry 2x6q coloured by chain, side view.
PDB entry 2x6q coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Structural insights on the new mechanism of trehalose synthesis by trehalose synthase TreT from Pyrococcus horikoshii.
Woo EJ, Ryu SI, Song HN, Jung TY, Yeon SM, Lee HA, Park BC, Park KH, Lee SB
J Mol Biol 404 247-59 (2010)
PMID: 20888836

Function and Biology Details

Reaction catalysed: 
NDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + NDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 48.24 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58762 (Residues: 2-415; Coverage: 100%)
Gene names: PH1035, treT
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 80.788Å b: 63.655Å c: 90.651Å
α: 90° β: 99.41° γ: 90°
R-values:
R R work R free
0.227 0.227 0.258
Expression system: Escherichia coli

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Citations

10 citation in other articles

Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens.
Greenfield et al. (2012)
9 more