› 2ws0

X-ray diffraction
2.1Å resolution

Semi-synthetic analogue of human insulin NMeAlaB26-insulin at pH 7.5

Released:
DOI: 10.2210/pdb2ws0/pdb
PDB entry 2ws0 coloured by chain, front view.
PDB entry 2ws0 coloured by chain, side view.
PDB entry 2ws0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues.
Jirácek J, Záková L, Antolíková E, Watson CJ, Turkenburg JP, Dodson GG, Brzozowski AM
Proc Natl Acad Sci U S A 107 1966-70 (2010)
PMID: 20133841

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MAA 1 x MAA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I4122
Unit cell:
a: 39.444Å b: 39.444Å c: 124.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.242 0.337
Expression system: Not provided

Quick links

Citations

4 review citations

Insulin receptor and cancer.
Belfiore et al. (2011)
3 more

1 mention without citation

The T2 structure of polycrystalline cubic human insulin.
Triandafillidis et al. (2023)