› 2wqm

X-ray diffraction
2.1Å resolution

Structure of apo human Nek7

Released:
DOI: 10.2210/pdb2wqm/pdb
PDB entry 2wqm coloured by chain, front view.
PDB entry 2wqm coloured by chain, side view.
PDB entry 2wqm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9.
Richards MW, O'Regan L, Mas-Droux C, Blot JM, Cheung J, Hoelder S, Fry AM, Bayliss R
Mol Cell 36 560-70 (2009)
PMID: 19941817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Nek7 Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TDX7 (Residues: 1-302; Coverage: 100%)
Gene name: NEK7
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I222
Unit cell:
a: 46.937Å b: 132.322Å c: 133.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Cell cycle regulation by the NEK family of protein kinases.
Fry et al. (2012)
13 more

12 mentions without citation

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
11 more