2wp8

X-ray diffraction
3Å resolution

yeast rrp44 nuclease

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155565 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Exosome complex component RRP45 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05636 (Residues: 1-305; Coverage: 100%)
Gene names: D9954.1, RRP45, YDR280W
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component SKI6 Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 27.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46948 (Residues: 1-246; Coverage: 100%)
Gene names: ECM20, G7587, RRP41, SKI6, YGR195W
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex exonuclease DIS3 Chain: J
Molecule details ›
Chain: J
Length: 977 amino acids
Theoretical weight: 111.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08162 (Residues: 25-1001; Coverage: 98%)
Gene names: DIS3, O2197, RRP44, YOL021C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 110.9Å b: 125.68Å c: 139.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.273
Expression system: Escherichia coli BL21(DE3)