› 2wg5

X-ray diffraction
2.1Å resolution

Proteasome-Activating Nucleotidase (PAN) N-domain (57-134) from Archaeoglobus fulgidus fused to GCN4

Released:
DOI: 10.2210/pdb2wg5/pdb
PDB entry 2wg5 coloured by chain, front view.
PDB entry 2wg5 coloured by chain, side view.
PDB entry 2wg5 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and activity of the N-terminal substrate recognition domains in proteasomal ATPases.
Djuranovic S, Hartmann MD, Habeck M, Ursinus A, Zwickl P, Martin J, Lupas AN, Zeth K
Mol Cell 34 580-90 (2009)
PMID: 19481487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Proteasome-activating nucleotidase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 109 amino acids
Theoretical weight: 12.41 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 249-274; Coverage: 9%)
  • Canonical: O28303 (Residues: 59-134; Coverage: 19%)
Gene names: AAS101, AAS3, AF_1976, ARG9, GCN4, YEL009C, pan
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 103.39Å b: 91.95Å c: 103.22Å
α: 90° β: 119.93° γ: 90°
R-values:
R R work R free
0.199 0.198 0.227
Expression system: Escherichia coli

Quick links

Citations

30 review citations

AAA+ proteases: ATP-fueled machines of protein destruction.
Sauer et al. (2011)
29 more

4 mentions without citation

AAA-ATPases in Protein Degradation.
Yedidi et al. (2017)
3 more