› 2w8n

X-ray diffraction
2Å resolution

The crystal structure of the oxidized form of human SSADH

Released:
DOI: 10.2210/pdb2w8n/pdb
PDB entry 2w8n coloured by chain, front view.
PDB entry 2w8n coloured by chain, side view.
PDB entry 2w8n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Redox-switch modulation of human SSADH by dynamic catalytic loop.
Kim YG, Lee S, Kwon OS, Park SY, Lee SJ, Park BJ, Kim KJ
EMBO J 28 959-68 (2009)
PMID: 19300440

Function and Biology Details

Reaction catalysed: 
Succinate semialdehyde + NAD(+) + H(2)O = succinate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinate-semialdehyde dehydrogenase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 52.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51649 (Residues: 49-535; Coverage: 91%)
Gene names: ALDH5A1, SSADH
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: F432
Unit cell:
a: 265.706Å b: 265.706Å c: 265.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.25
Expression system: Escherichia coli

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Citations

6 review citations

Thinking outside the cleft to understand synaptic activity: contribution of the cystine-glutamate antiporter (System xc-) to normal and pathological glutamatergic signaling.
Bridges et al. (2012)
5 more

9 mentions without citation

Aldehyde dehydrogenases: from eye crystallins to metabolic disease and cancer stem cells.
Vasiliou et al. (2013)
8 more