› 2w8c

X-ray diffraction
1.8Å resolution

Plastocyanin variant with N-terminal Methionine - closed structure

Released:
DOI: 10.2210/pdb2w8c/pdb
PDB entry 2w8c coloured by chain, front view.
PDB entry 2w8c coloured by chain, side view.
PDB entry 2w8c coloured by chain, top view.
Source organism: Phormidium laminosum
Primary publication:
Metal-mediated self-assembly of a beta-sandwich protein.
Crowley PB, Matias PM, Khan AR, Roessle M, Svergun DI
Chemistry 15 12672-80 (2009)
PMID: 19834935

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-175980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plastocyanin Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.56 KDa
Source organism: Phormidium laminosum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q51883 (Residues: 35-139; Coverage: 100%)
Gene name: petE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: R3
Unit cell:
a: 55.74Å b: 55.74Å c: 197.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.173 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Protein dielectric constants determined from NMR chemical shift perturbations.
Kukic et al. (2013)
1 more