› 2w57

X-ray diffraction
2.6Å resolution

Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals structural rearrangement of the DNA-binding domains

Released:
DOI: 10.2210/pdb2w57/pdb
PDB entry 2w57 coloured by chain, front view.
PDB entry 2w57 coloured by chain, side view.
PDB entry 2w57 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals insights into metal co-ordination.
Sheikh MA, Taylor GL
Mol Microbiol 72 1208-20 (2009)
PMID: 19400801

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric uptake regulation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.94 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C6C8 (Residues: 1-150; Coverage: 100%)
Gene names: VC_2106, fur
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 88.88Å b: 88.88Å c: 85.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
16 more

15 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
14 more