› 2vyr

X-ray diffraction
2Å resolution

Structure of human MDM4 N-terminal domain bound to a single domain antibody

Released:
DOI: 10.2210/pdb2vyr/pdb
PDB entry 2vyr coloured by chain, front view.
PDB entry 2vyr coloured by chain, side view.
PDB entry 2vyr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human MDM4 N-terminal domain bound to a single-domain antibody.
Yu GW, Vaysburd M, Allen MD, Settanni G, Fersht AR
J Mol Biol 385 1578-89 (2009)
PMID: 19084022

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208050 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Mdm4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 101 amino acids
Theoretical weight: 11.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15151 (Residues: 16-116; Coverage: 21%)
Gene names: MDM4, MDMX
Structure domains: SWIB/MDM2 domain
HUMAN SINGLE DOMAIN ANTIBODY Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 153 amino acids
Theoretical weight: 17.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 79.823Å b: 115.2Å c: 99.368Å
α: 90° β: 104.86° γ: 90°
R-values:
R R work R free
0.207 0.207 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Antigen recognition by single-domain antibodies: structural latitudes and constraints.
Henry et al. (2018)
5 other articles

6 mentions without citation

Wrecked regulation of intrinsically disordered proteins in diseases: pathogenicity of deregulated regulators.
Uversky VN. (2014)
5 more