› 2vvo

X-ray diffraction
1.85Å resolution

Crystal structure of Mycobacterium tuberculosis ribose-5-phosphate isomerase B in complex with alpha d-allose 6-phosphate

Released:
DOI: 10.2210/pdb2vvo/pdb
PDB entry 2vvo coloured by chain, front view.
PDB entry 2vvo coloured by chain, side view.
PDB entry 2vvo coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
D-ribose-5-phosphate isomerase B from Escherichia coli is also a functional D-allose-6-phosphate isomerase, while the Mycobacterium tuberculosis enzyme is not.
Roos AK, Mariano S, Kowalinski E, Salmon L, Mowbray SL
J Mol Biol 382 667-79 (2008)
PMID: 18640127

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase B Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 162 amino acids
Theoretical weight: 17.3 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WKD7 (Residues: 1-162; Coverage: 100%)
Gene names: MTV008.21c, Rv2465c, rpiB
Sequence domains: Ribose/Galactose Isomerase
Structure domains: Sugar-phosphate isomerase, RpiB/LacA/LacB

Ligands and Environments

1 bound ligand:
Ligand A6P 5 x A6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 152.744Å b: 92.501Å c: 60.967Å
α: 90° β: 98.92° γ: 90°
R-values:
R R work R free
0.162 0.16 0.196
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst.
Tang et al. (2021)
14 other articles

3 mentions without citation

A coarse-grained elastic network atom contact model and its use in the simulation of protein dynamics and the prediction of the effect of mutations.
Frappier et al. (2014)
2 more