› 2vs3

X-ray diffraction
2.2Å resolution

THE BINDING OF UDP-GALACTOSE BY AN ACTIVE SITE MUTANT OF alpha-1,3 GALACTOSYLTRANSFERASE (alpha3GT)

Released:
DOI: 10.2210/pdb2vs3/pdb
PDB entry 2vs3 coloured by chain, front view.
PDB entry 2vs3 coloured by chain, side view.
PDB entry 2vs3 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of UDP-galactose binding by alpha-1,3-galactosyltransferase (alpha3GT): role of negative charge on aspartic acid 316 in structure and activity.
Tumbale P, Jamaluddin H, Thiyagarajan N, Brew K, Acharya KR
Biochemistry 47 8711-8 (2008)
PMID: 18651752

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 34.09 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, GAL
Carbohydrate polymer
2 bound ligands:
Ligand UDP 2 x UDP
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P21
Unit cell:
a: 45.116Å b: 94.38Å c: 94.94Å
α: 90° β: 99.3° γ: 90°
R-values:
R R work R free
0.215 0.215 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

A molecular description of cellulose biosynthesis.
McNamara et al. (2015)
1 more

3 mentions without citation

Structural insights into the recognition of substrates and activators by the OSR1 kinase.
Villa et al. (2007)
2 more