› 2vr2

X-ray diffraction
2.8Å resolution

Human Dihydropyrimidinase

Released:
DOI: 10.2210/pdb2vr2/pdb
PDB entry 2vr2 coloured by chain, front view.
PDB entry 2vr2 coloured by chain, side view.
PDB entry 2vr2 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Welin M, Karlberg T, Andersson J, Arrowsmith CH, Berglund H, Busam RD, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Herman MD, Johansson I, Kallas A, Kotenyova T, Lehtio L, Moche M, Nilsson ME, Nyman T, Persson C, Sagemark J, Svensson L, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Wikstrom M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
5,6-dihydrouracil + H(2)O = 3-ureidopropanoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropyrimidinase Chain: A
Molecule details ›
Chain: A
Length: 541 amino acids
Theoretical weight: 59.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14117 (Residues: 1-519; Coverage: 100%)
Gene name: DPYS
Sequence domains: Amidohydrolase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6422
Unit cell:
a: 89.65Å b: 89.65Å c: 219.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.251
Expression system: Escherichia coli BL21(DE3)

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