› 2vki

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoinositide-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 17.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15530 (Residues: 409-556; Coverage: 27%)
Gene names: PDK1, PDPK1
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 34.009Å b: 67.057Å c: 67.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
16 more

3 mentions without citation

Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance.
Bayascas et al. (2008)
2 more