› 2vaz

Electron Microscopy
10Å resolution

Model of the S15-mRNA complex fitted into the cryo-EM map of the 70S entrapment complex.

Released:
DOI: 10.2210/pdb2vaz/pdb
PDB entry 2vaz coloured by chain, front view.
PDB entry 2vaz coloured by chain, side view.
PDB entry 2vaz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structured mRNAs regulate translation initiation by binding to the platform of the ribosome.
Marzi S, Myasnikov AG, Serganov A, Ehresmann C, Romby P, Yusupov M, Klaholz BP
Cell 130 1019-31 (2007)
PMID: 17889647
Related structures: EMD-1391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142390 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Small ribosomal subunit protein uS15 Chain: F
Molecule details ›
Chain: F
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
RPSO MRNA OPERATOR Chain: A
Molecule details ›
Chain: A
Length: 185 nucleotides
Theoretical weight: 59.43 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: Ribosomal S15 leader

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 10Å
Relevant EMDB volumes: EMD-1391
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

Regulation of translation initiation by RNA binding proteins.
Babitzke et al. (2009)
16 more

3 mentions without citation

Towards deciphering the principles underlying an mRNA recognition code.
Serganov et al. (2008)
2 more