2v7q

X-ray diffraction
2.1Å resolution

The structure of F1-ATPase inhibited by I1-60HIS, a monomeric form of the inhibitor protein, IF1.

Released:
Source organism: Bos taurus
Primary publication:
How the regulatory protein, IF(1), inhibits F(1)-ATPase from bovine mitochondria.
Proc Natl Acad Sci U S A 104 15671-6 (2007)
PMID: 17895376

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-133615 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 482 amino acids
Theoretical weight: 51.76 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 47-528; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chain: G
Molecule details ›
Chain: G
Length: 272 amino acids
Theoretical weight: 30.19 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-297; Coverage: 91%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase
Structure domains:
ATP synthase subunit delta, mitochondrial Chain: H
Molecule details ›
Chain: H
Length: 146 amino acids
Theoretical weight: 15.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05630 (Residues: 23-168; Coverage: 87%)
Gene names: ATP5D, ATP5F1D
Sequence domains:
Structure domains:
ATP synthase subunit epsilon, mitochondrial Chain: I
Molecule details ›
Chain: I
Length: 50 amino acids
Theoretical weight: 5.66 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05632 (Residues: 2-51; Coverage: 98%)
Gene names: ATP5E, ATP5F1E
Sequence domains: Mitochondrial ATP synthase epsilon chain
Structure domains: ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial
ATPase inhibitor, mitochondrial Chain: J
Molecule details ›
Chain: J
Length: 66 amino acids
Theoretical weight: 7.46 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01096 (Residues: 26-85; Coverage: 55%)
Gene names: ATP5IF1, ATPI, ATPIF1
Sequence domains: Mitochondrial ATPase inhibitor, IATP
Structure domains: Single helix bin

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 262.534Å b: 103.271Å c: 135.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.245
Expression system: Escherichia coli BL21(DE3)