› 2v51

X-ray diffraction
2.35Å resolution

Structure of MAL-RPEL1 complexed to actin

Released:
DOI: 10.2210/pdb2v51/pdb
PDB entry 2v51 coloured by chain, front view.
PDB entry 2v51 coloured by chain, side view.
PDB entry 2v51 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL.
Mouilleron S, Guettler S, Langer CA, Treisman R, McDonald NQ
EMBO J 27 3198-208 (2008)
PMID: 19008859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159319 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: B, D
Molecule details ›
Chains: B, D
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Myocardin-related transcription factor A Chains: E, F
Molecule details ›
Chains: E, F
Length: 32 amino acids
Theoretical weight: 3.78 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q8K4J6 (Residues: 14-41; Coverage: 3%)
Gene names: Bsac, Mal, Mkl1, Mrtfa
Sequence domains: RPEL repeat

Ligands and Environments

5 bound ligands:
Ligand ATP 2 x ATP
Ligand CA 2 x CA
Ligand LAB 2 x LAB
Ligand PEG 2 x PEG
Ligand SCN 4 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 53.092Å b: 59.071Å c: 166.203Å
α: 90° β: 90.06° γ: 90°
R-values:
R R work R free
0.186 0.183 0.248
Expression system: Not provided

Quick links

Citations

20 review citations

Linking actin dynamics and gene transcription to drive cellular motile functions.
Olson et al. (2010)
19 more

2 mentions without citation

Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL.
Mouilleron et al. (2008)
1 more