› 2v4d

X-ray diffraction
3.2Å resolution

Re-refinement of MexA adaptor protein

Released:
DOI: 10.2210/pdb2v4d/pdb
PDB entry 2v4d coloured by chain, front view.
PDB entry 2v4d coloured by chain, side view.
PDB entry 2v4d coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The assembled structure of a complete tripartite bacterial multidrug efflux pump.
Symmons MF, Bokma E, Koronakis E, Hughes C, Koronakis V
Proc Natl Acad Sci U S A 106 7173-8 (2009)
PMID: 19342493

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance protein MexA Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Length: 360 amino acids
Theoretical weight: 38.62 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52477 (Residues: 24-383; Coverage: 100%)
Gene names: PA0425, mexA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 130.551Å b: 183.585Å c: 213.314Å
α: 90° β: 107.38° γ: 90°
R-values:
R R work R free
0.24 0.239 0.264
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
45 more

22 mentions without citation

RND-type drug efflux pumps from Gram-negative bacteria: molecular mechanism and inhibition.
Venter et al. (2015)
21 more