› 2v25

X-ray diffraction
1.49Å resolution

Structure of the Campylobacter jejuni antigen Peb1A, an aspartate and glutamate receptor with bound aspartate

Released:
DOI: 10.2210/pdb2v25/pdb
PDB entry 2v25 coloured by chain, front view.
PDB entry 2v25 coloured by chain, side view.
PDB entry 2v25 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
A bacterial virulence factor with a dual role as an adhesin and a solute-binding protein: the crystal structure at 1.5 A resolution of the PEB1a protein from the food-borne human pathogen Campylobacter jejuni.
Müller A, León-Kempis Mdel R, Dodson E, Wilson KS, Wilkinson AJ, Kelly DJ
J Mol Biol 372 160-71 (2007)
PMID: 17631313

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major cell-binding factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 28.22 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0P9X8 (Residues: 1-259; Coverage: 100%)
Gene names: Cj0921c, peb1A
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand ASP 2 x ASP
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 53.272Å b: 75.868Å c: 61.708Å
α: 90° β: 103.31° γ: 90°
R-values:
R R work R free
0.165 0.163 0.185
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Host epithelial cell invasion by Campylobacter jejuni: trigger or zipper mechanism?
O Cróinín et al. (2012)
3 more

6 mentions without citation

An Aspartate-Specific Solute-Binding Protein Regulates Protein Kinase G Activity To Control Glutamate Metabolism in Mycobacteria.
Bhattacharyya et al. (2018)
5 more