› 2tnf

X-ray diffraction
1.4Å resolution

1.4 A RESOLUTION STRUCTURE OF MOUSE TUMOR NECROSIS FACTOR, TOWARDS MODULATION OF ITS SELECTIVITY AND TRIMERISATION

Released:
DOI: 10.2210/pdb2tnf/pdb
PDB entry 2tnf coloured by chain, front view.
PDB entry 2tnf coloured by chain, side view.
PDB entry 2tnf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization.
Baeyens KJ, De Bondt HL, Raeymaekers A, Fiers W, De Ranter CJ
Acta Crystallogr D Biol Crystallogr 55 772-8 (1999)
PMID: 10089307

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 156 amino acids
Theoretical weight: 17.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06804 (Residues: 80-235; Coverage: 66%)
Gene names: Tnf, Tnfa, Tnfsf2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P1
Unit cell:
a: 48.214Å b: 48.3Å c: 51.095Å
α: 114.81° β: 103.65° γ: 91.05°
R-values:
R R work R free
0.201 0.201 0.235
Expression system: Escherichia coli

Quick links

Citations

5 review citations

TNF alpha and the TNF receptor superfamily: structure-function relationship(s).
Idriss et al. (2000)
4 more

23 mentions without citation

Subversion of cytokine networks by virally encoded decoy receptors.
Epperson et al. (2012)
22 more