› 2riq

X-ray diffraction
1.7Å resolution

Crystal Structure of the Third Zinc-binding domain of human PARP-1

Released:
DOI: 10.2210/pdb2riq/pdb
PDB entry 2riq coloured by chain, front view.
PDB entry 2riq coloured by chain, side view.
PDB entry 2riq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordinates DNA-dependent enzyme activation.
Langelier MF, Servent KM, Rogers EE, Pascal JM
J Biol Chem 283 4105-14 (2008)
PMID: 18055453

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase 1, processed C-terminus Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 18.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09874 (Residues: 216-366; Coverage: 15%)
Gene names: ADPRT, PARP1, PPOL
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand EOH 1 x EOH
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 71.841Å b: 85.652Å c: 67.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.23
Expression system: Escherichia coli

Quick links

Citations

38 review citations

PARP inhibition: PARP1 and beyond.
Rouleau et al. (2010)
37 more

7 mentions without citation

The PARP side of the nucleus: molecular actions, physiological outcomes, and clinical targets.
Krishnakumar et al. (2010)
6 more