› 2rhl

X-ray diffraction
2.45Å resolution

Synthetic Gene Encoded Bacillus Subtilis FtsZ NCS Dimer with Bound GDP

Released:
DOI: 10.2210/pdb2rhl/pdb
PDB entry 2rhl coloured by chain, front view.
PDB entry 2rhl coloured by chain, side view.
PDB entry 2rhl coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
Raymond A, Lovell S, Lorimer D, Walchli J, Mixon M, Wallace E, Thompkins K, Archer K, Burgin A, Stewart L
BMC Biotechnol 9 37 (2009)
PMID: 19383143

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 34.05 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P17865 (Residues: 12-315; Coverage: 80%)
Gene names: BSU15290, ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 82.294Å b: 97.577Å c: 135.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression system: Escherichia coli

Quick links

Citations

6 review citations

High-throughput protein purification and quality assessment for crystallization.
Kim et al. (2011)
5 more

13 mentions without citation

At the Heart of Bacterial Cytokinesis: The Z Ring.
Du et al. (2019)
12 more