› 2r8k

X-ray diffraction
3.3Å resolution

Structure of the Eukaryotic DNA Polymerase eta in complex with 1,2-d(GpG)-cisplatin containing DNA

Released:
DOI: 10.2210/pdb2r8k/pdb
PDB entry 2r8k coloured by chain, front view.
PDB entry 2r8k coloured by chain, side view.
PDB entry 2r8k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA polymerase eta.
Alt A, Lammens K, Chiocchini C, Lammens A, Pieck JC, Kuch D, Hopfner KP, Carell T
Science 318 967-70 (2007)
PMID: 17991862

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115828 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chains: A, B
Molecule details ›
Chains: A, B
Length: 554 amino acids
Theoretical weight: 62.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04049 (Residues: 1-531; Coverage: 84%)
Gene names: DBH1, RAD30, YDR419W
Sequence domains: impB/mucB/samB family
Structure domains:
5'-D(*DGP*DTP*DGP*DGP*DTP*DGP*DAP*DGP*DC)-3' Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 nucleotides
Theoretical weight: 2.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(P*DGP*DGP*DCP*DTP*DCP*DAP*DCP*DCP*DAP*DC)-3' Chains: T, U
Molecule details ›
Chains: T, U
Length: 10 nucleotides
Theoretical weight: 2.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CPT 2 x CPT
Ligand CA 4 x CA
Ligand DTP 2 x DTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 103.74Å b: 103.74Å c: 292.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Molecular mechanisms of cisplatin resistance.
Galluzzi et al. (2012)
24 more

3 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
2 more