› 2r87

X-ray diffraction
2.3Å resolution

Crystal structure of PurP from Pyrococcus furiosus complexed with ADP

Released:
DOI: 10.2210/pdb2r87/pdb
PDB entry 2r87 coloured by chain, front view.
PDB entry 2r87 coloured by chain, side view.
PDB entry 2r87 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.
Zhang Y, White RH, Ealick SE
Biochemistry 47 205-17 (2008)
PMID: 18069798

Function and Biology Details

Reaction catalysed: 
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 334 amino acids
Theoretical weight: 38.35 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U0R7 (Residues: 1-334; Coverage: 100%)
Gene names: PF1517, purP
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 19 x PO4
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 76.095Å b: 127.222Å c: 121.673Å
α: 90° β: 102.85° γ: 90°
R-values:
R R work R free
0.203 0.201 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
2 more

1 mention without citation

Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.
Zhang et al. (2008)