› 2qom

X-ray diffraction
2.66Å resolution

The crystal structure of the E.coli EspP autotransporter Beta-domain.

Released:
DOI: 10.2210/pdb2qom/pdb
PDB entry 2qom coloured by chain, front view.
PDB entry 2qom coloured by chain, side view.
PDB entry 2qom coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.
Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK
Nat Struct Mol Biol 14 1214-20 (2007)
PMID: 17994105

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter protein EspP translocator Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 31.34 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7BSW5 (Residues: 1024-1300; Coverage: 22%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Autotransporter beta-domain
Structure domains: Autotransporter beta-domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 84.951Å b: 53.298Å c: 102.418Å
α: 90° β: 103.57° γ: 90°
R-values:
R R work R free
0.26 0.258 0.284
Expression system: Escherichia coli BL21

Quick links

Citations

28 review citations

From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis.
Leyton et al. (2012)
27 more

22 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
21 more