› 2qlt

X-ray diffraction
1.6Å resolution

Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb2qlt/pdb
PDB entry 2qlt coloured by chain, front view.
PDB entry 2qlt coloured by chain, side view.
PDB entry 2qlt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Tan K, Evdokimova E, Kudritska M, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
sn-glycerol 1-phosphate + H(2)O = glycerol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-1-phosphate phosphohydrolase 1 Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P41277 (Residues: 1-250; Coverage: 100%)
Gene names: GPP1, RHR2, YIL053W
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand HG 4 x HG
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand SO4 4 x SO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 39.636Å b: 55.578Å c: 97.748Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.195
Expression system: Escherichia coli BL21

Quick links