› 2qiw

X-ray diffraction
1.8Å resolution

Crystal structure of a putative phosphoenolpyruvate phosphonomutase (ncgl1015, cgl1060) from corynebacterium glutamicum atcc 13032 at 1.80 A resolution

Released:
DOI: 10.2210/pdb2qiw/pdb
PDB entry 2qiw coloured by chain, front view.
PDB entry 2qiw coloured by chain, side view.
PDB entry 2qiw coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
1-carboxyvinyl carboxyphosphonate = 3-(hydrohydroxyphosphoryl)pyruvate + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PEP phosphonomutase and related enzymes Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 26.9 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NRI8 (Residues: 1-254; Coverage: 100%)
Gene name: Cgl1060
Sequence domains: Phosphoenolpyruvate phosphomutase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 2 x SO4
Ligand UNL 1 x UNL
Ligand GOL 11 x GOL
Ligand CL 1 x CL
Ligand P6G 1 x P6G
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 82.52Å b: 94.56Å c: 107.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.168
Expression system: Escherichia coli

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