› 2qgn

X-ray diffraction
2.4Å resolution

Crystal structure of tRNA isopentenylpyrophosphate transferase (BH2366) from Bacillus halodurans, Northeast Structural Genomics Consortium target BhR41.

Released:
DOI: 10.2210/pdb2qgn/pdb
PDB entry 2qgn coloured by chain, front view.
PDB entry 2qgn coloured by chain, side view.
PDB entry 2qgn coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Forouhar F, Neely H, Abashidze M, Seetharaman J, Shastry R, Janjua H, Cunningham K, Ma L-C, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-(3-methylbut-2-en-1-yl)-adenine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA dimethylallyltransferase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 37.4 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KAC3 (Residues: 1-314; Coverage: 100%)
Gene names: BH2366, miaA
Sequence domains: IPP transferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P61
Unit cell:
a: 50.855Å b: 50.855Å c: 204.697Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.277
Expression system: Escherichia coli

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