› 2qby

X-ray diffraction
3.35Å resolution

Crystal structure of a heterodimer of Cdc6/Orc1 initiators bound to origin DNA (from S. solfataricus)

Released:
DOI: 10.2210/pdb2qby/pdb
PDB entry 2qby coloured by chain, front view.
PDB entry 2qby coloured by chain, side view.
PDB entry 2qby coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex.
Dueber EL, Corn JE, Bell SD, Berger JM
Science 317 1210-3 (2007)
PMID: 17761879

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115797 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
ORC1-type DNA replication protein 1 Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 43.92 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980N4 (Residues: 15-397; Coverage: 97%)
Gene names: SSO0257, cdc6-1
Sequence domains:
Structure domains:
ORC1-type DNA replication protein 3 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 43.47 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97WM8 (Residues: 14-394; Coverage: 97%)
Gene names: SSO2184, cdc6-3
Sequence domains:
Structure domains:
DNA (33-MER) Chain: C
Molecule details ›
Chain: C
Length: 33 nucleotides
Theoretical weight: 10.2 KDa
DNA (33-MER) Chain: D
Molecule details ›
Chain: D
Length: 33 nucleotides
Theoretical weight: 10.09 KDa

Ligands and Environments

4 bound ligands:
Ligand SPD 2 x SPD
Ligand K 1 x K
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 77.649Å b: 199.143Å c: 213.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.269
Expression system: Escherichia coli

Quick links

Citations

35 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
34 more

21 mentions without citation

A structural framework for replication origin opening by AAA+ initiation factors.
Duderstadt et al. (2013)
20 more