› 2q7r

X-ray diffraction
4Å resolution

Crystal structure of human FLAP with an iodinated analog of MK-591

Released:
DOI: 10.2210/pdb2q7r/pdb
PDB entry 2q7r coloured by chain, front view.
PDB entry 2q7r coloured by chain, side view.
PDB entry 2q7r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of inhibitor-bound human 5-lipoxygenase-activating protein.
Ferguson AD, McKeever BM, Xu S, Wisniewski D, Miller DK, Yamin TT, Spencer RH, Chu L, Ujjainwalla F, Cunningham BR, Evans JF, Becker JW
Science 317 510-2 (2007)
PMID: 17600184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arachidonate 5-lipoxygenase-activating protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 161 amino acids
Theoretical weight: 18.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20292 (Residues: 1-161; Coverage: 100%)
Gene names: ALOX5AP, FLAP
Sequence domains: MAPEG family
Structure domains: Membrane associated eicosanoid/glutathione metabolism-like domain

Ligands and Environments

1 bound ligand:
Ligand 3CS 6 x 3CS
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4212
Unit cell:
a: 180.66Å b: 180.66Å c: 139.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Leukotrienes.
Peters-Golden et al. (2007)
27 more

10 mentions without citation

Vertebrate membrane proteins: structure, function, and insights from biophysical approaches.
Müller et al. (2008)
9 more