› 2pub

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES

Released:
DOI: 10.2210/pdb2pub/pdb
PDB entry 2pub coloured by chain, front view.
PDB entry 2pub coloured by chain, side view.
PDB entry 2pub coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices.
Schumacher MA, Choi KY, Zalkin H, Brennan RG
Science 266 763-70 (1994)
PMID: 7973627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115779 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor PurR Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0ACP7 (Residues: 2-341; Coverage: 100%)
Gene names: JW1650, b1658, purR
Sequence domains:
Structure domains:
DNA (5'-D(*TP*AP*CP*GP*CP*AP*AP*AP*CP*GP*TP*TP*TP*GP*CP*GP*T )-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 175.81Å b: 94.87Å c: 81.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 not available
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

37 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
36 more

2 mentions without citation

Novel sequence-based method for identifying transcription factor binding sites in prokaryotic genomes.
Sahota et al. (2010)
1 more