› 2prt

X-ray diffraction
3.15Å resolution

Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA

Released:
DOI: 10.2210/pdb2prt/pdb
PDB entry 2prt coloured by chain, front view.
PDB entry 2prt coloured by chain, side view.
PDB entry 2prt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Wilms tumor suppressor protein zinc finger domain bound to DNA.
Stoll R, Lee BM, Debler EW, Laity JH, Wilson IA, Dyson HJ, Wright PE
J Mol Biol 372 1227-45 (2007)
PMID: 17716689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115777 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19544 (Residues: 318-438; Coverage: 26%)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*CP*GP*CP*GP*GP*GP*GP*GP*CP*GP*TP*CP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*GP*AP*CP*GP*CP*CP*CP*CP*CP*GP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P6122
Unit cell:
a: 86.879Å b: 86.879Å c: 171.202Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Wilms' tumours: about tumour suppressor genes, an oncogene and a chameleon gene.
Huff V. (2011)
7 more

13 mentions without citation

Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence.
Hashimoto et al. (2014)
12 more