› 2pr9

X-ray diffraction
2.51Å resolution

Mu2 adaptin subunit (AP50) of AP2 adaptor (second domain), complexed with GABAA receptor-gamma2 subunit-derived internalization peptide DEEYGYECL

Released:
DOI: 10.2210/pdb2pr9/pdb
PDB entry 2pr9 coloured by chain, front view.
PDB entry 2pr9 coloured by chain, side view.
PDB entry 2pr9 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit.
Kittler JT, Chen G, Kukhtina V, Vahedi-Faridi A, Gu Z, Tretter V, Smith KR, McAinsh K, Arancibia-Carcamo IL, Saenger W, Haucke V, Yan Z, Moss SJ
Proc Natl Acad Sci U S A 105 3616-21 (2008)
PMID: 18305175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148390 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AP-2 complex subunit mu Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 158-435; Coverage: 64%)
Gene name: Ap2m1
Sequence domains: Adaptor complexes medium subunit family
Structure domains: Mu homology domain, subdomain B
Gamma-aminobutyric acid receptor subunit gamma-2 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.24 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P18508 (Residues: 400-409; Coverage: 2%)
Gene name: Gabrg2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P64
Unit cell:
a: 126.3Å b: 126.3Å c: 74.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

29 review citations

GABA(A) receptor trafficking and its role in the dynamic modulation of neuronal inhibition.
Jacob et al. (2008)
28 more

3 mentions without citation

Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit.
Kittler et al. (2008)
2 more