› 2pe5

X-ray diffraction
3.5Å resolution

Crystal Structure of the Lac Repressor bound to ONPG in repressed state

Released:
DOI: 10.2210/pdb2pe5/pdb
PDB entry 2pe5 coloured by chain, front view.
PDB entry 2pe5 coloured by chain, side view.
PDB entry 2pe5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis of lac repressor bound to allosteric effectors.
Daber R, Stayrook S, Rosenberg A, Lewis M
J Mol Biol 370 609-19 (2007)
PMID: 17543986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115758 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Lactose operon repressor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 330 amino acids
Theoretical weight: 35.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03023 (Residues: 2-331; Coverage: 92%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains:
DNA (5'-D(*DAP*DAP*DTP*DTP*DGP*DTP*DGP*DAP*DGP*DCP*DGP*DCP*DTP*DCP*DAP*DCP*DAP*DAP*DTP*DT)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand 145 3 x 145
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R32
Unit cell:
a: 253.08Å b: 253.08Å c: 203.247Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.233 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
4 more

8 mentions without citation

Unified rational protein engineering with sequence-based deep representation learning.
Alley et al. (2019)
7 more