› 2p7t

X-ray diffraction
2.05Å resolution

Crystal Structure of KcsA mutant

Released:
DOI: 10.2210/pdb2p7t/pdb
PDB entry 2p7t coloured by chain, front view.
PDB entry 2p7t coloured by chain, side view.
PDB entry 2p7t coloured by chain, top view.
Source organisms:
Entry authors: Cordero-Morales JF, Vishwanath J, Lewis A, Valeria VR, Cortes DM, Roux B, Perozo E

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
hetero dimer
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209462 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB-A Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
FAB-B Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 10.94 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand F09 1 x F09
Ligand K 6 x K
Ligand 1EM 1 x 1EM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I4
Unit cell:
a: 156.339Å b: 156.339Å c: 76.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.234
Expression system: Escherichia coli

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